Paper electrophoresis as a quantitative method. Serum proteins

Quantitative electrophoresis of serum proteins on paper.

CONSIDERABLE NUMBER OF STUDIES devoted to the quantitative evaluation of paper-electrophoretic patterns have left doubt as to the value of the method as an accurate quantitative procedure. Some workers feel that paper electrophoresis cannot be expected to equal the reproducibility of the moving-boundary technic by virtue of its relative crudeness as compared with the sensitive nature of refract...

Paper electrophoresis as a quantitative method: the staining of serum lipoproteins.

Electrophoresis of serum on filter paper followed by staining 3 of the lipoprotein fractions with lipid dyes is technically the simplest method for the separation and estimation of serum lipoproteins. A number of procedures for the staining of separated lipoproteins on paper with various lipid dyes have appeared (1-8), but the only comprehensive consideration of the quantitative aspects of dye ...

Paper electrophoresis of serum proteins in children.

Since the description of paper electrophoresis in 1950 by Cremer and Tiselius and by Durrum among others, considerable interest has been shown in this cheap and relatively quick method of separating the various protein fractions. The serum proteins move through the fine cellulose mesh of the paper under the influence of an electric current, the separation of the various fractions being brought ...

Quantitative analysis of serum proteins separated by capillary electrophoresis.

The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samp...

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis